Abstract

Agricultural management practices control soil organic carbon (SOC) content in croplands. Long-term cropping system experiments offer a great opportunity to understand the magnitude and direction of SOC change in response to management practices. Such information is very limited from the southeastern US, a region with warm and humid climatic conditions that typically favor SOC decomposition over accumulation. Therefore, this study was conducted to assess the effect of 39 years of chisel plow (CP), disc plow (DP), moldboard plow (MP), no-tillage (NT), NT with winter wheat (Triticum aestivum L.) cover crop (NTW), and NT with wheat-soybean (Glycine max L.) double crop (NTWD) on total SOC and SOC fractions including permanganate oxidizable C (POXC), water extractable C (WEC), resistant C (RC), and aggregate-associated SOC in a continuous soybean system. Additionally, aggregate size distribution, mean weight diameter (MWD), and wet aggregate stability (WAS) were determined. Results showed that NTW and NTWD significantly increased SOC and POXC compared to MP with mean SOC (g kg−1 soil) of 12.2 (NTW) ≥10.9 (NTWD) >7.2 (MP) and mean POXC (mg kg−1 soil) of 465 (NTWD) ≥418 (NTW) >252 (MP). The WEC and RC fractions did not differ among treatments. Across the treatments, the greatest aggregate-associated SOC concentration was found in microaggregates (0.053–0.25 mm) and the lowest in clay- and silt-size particles (<0.053 mm). Additionally, WAS under NT systems was significantly higher (45.5–52.3 %) than under tilled treatments (21.9–29.1 %). Total SOC correlated significantly with POXC (r = 0.68, p < 0.01), RC (r = 0.46, p < 0.05), WAS (r = 0.65, p < 0.01), and aggregate-associated SOC concentrations (r > 0.6, p < 0.01). Overall, this study revealed that NT enhanced SOC and POXC accumulation and macroaggregation compared to tilled treatments. Cover cropping and double cropping further improved SOC accumulation. In conclusion, long-term adoption of different tillage intensities can strongly alter SOC dynamics in bulk soil and aggregates under continuous soybean production systems of the southeastern US.

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