Soil microbial community structure and environmental effects of serpentine weathering under different vegetative covers in the serpentine mining area of Donghai County, China

Abstract

The use of serpentine biological weathering to capture atmospheric CO2 has attracted much attention. In the long-term mining activities in a serpentine mining area, a large amount of serpentine powder diffused into the surrounding forest and farmland soil. The study of the serpentine weathering in soils of different vegetative covers and the composition characteristics of soil carbonate has important implications for understanding the serpentine weathering and carbon sequestration under natural conditions. The microbial diversity on exposed rock serpentine surfaces and soil under different vegetative covers in the serpentine mining area in Donghai County, China was investigated by high-throughput sequencing technology, and the characteristics of serpentine weathering and soil carbonate in related area were also explored by XRF, XRD, SEM-EDS, and chemical analysis methods. The results showed that the richness and uniformity of the bacteria species community increased significantly with the increasing complexity of plant groups covering the rock surface, but the species richness and uniformity of fungi showed an overall declining trend. Furthermore, high‑magnesium calcite (HMC) is ubiquitous on the exposed rock surface and the soil under different vegetative covers in this area. Based on these results, combined with the verification test results of HMC fixed heavy metal ions, the model of serpentine weathering in serpentine mining soil to synthesize carbonate and fix heavy metal ions was developed. That is, with the increase in the degree of rock weathering and the colonization of plants, the soil and plants seem to shape jointly a relatively stable microbial community structure adapted to the environment of the serpentine mining area, which promotes the serpentine weathering coupled with the formation of HMC and immobilization of metal ions in the serpentine soil. This study provides a theoretical basis for the serpentine bio-weathering in the mine area to capture atmospheric CO2.