Sodium–hydrogen exchangers and sodium–bicarbonate co-transporters: ontogeny of protein expression in the rat brain

Abstract

We used western blotting to examine the developmental profiles (at embryonic day 16 and postnatal days 1, 13, 23, 33 and 105) of protein expression for three sodium–hydrogen exchanger isoforms (1, 2 and 4) and for a sodium–bicarbonate co-transporter in three CNS regions (cortex, cerebellum and brainstem–diencephalon). In microsomal preparations, sodium–hydrogen exchanger isoform 1 and sodium–bicarbonate co-transporter protein expression in the CNS increases gradually from embryonic day 16 (25–40% of the adult level) to postnatal day 105. In contrast, sodium–hydrogen exchanger isoform 2 and 4 expression reaches a maximum (three to 20 times the adult level) at around three to four weeks of age. There is significant regional heterogeneity in the expression of sodium–hydrogen exchanger and sodium–bicarbonate co-transporter proteins in the rat CNS. Sodium–hydrogen exchanger isoform 1 was highly expressed in the brainstem–diencephalon, whereas the sodium–bicarbonate co-transporter was robustly expressed in the cerebellum and brainstem–diencephalon. These data indicate that the expression of sodium–hydrogen exchanger and sodium–bicarbonate co-transporter proteins varies as a function of both development and specific brain region.