Abstract
AbstractA metod is described for obtaining a sample‐stacking effect before sodium dodecyl sulfate‐agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide‐agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be separated, according to the molecular weight of their subunits, in 15 % w/w agarose gels. The new gel system is versatile and may offer advantages over sodium dodecyl sulfate ‐ polyacrylamide gel electrophoresis in such applications as protein elution or enzyme renaturation.
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