Abstract
We aimed to clarify the molecular mechanism of lncRNA SNHG1 in regulating the OSCC process. Clinical samples of OSCC were collected for detecting the differential level of SNHG1 by qRT-PCR. Pathological indexes of OSCC patients were analyzed for uncovering the prognostic value of SNHG1. The interaction between SNHG1 and miR-145-5p was assessed through the bioinformatics method and dual-luciferase reporter assay. Their coregulation on proliferative and migratory functions of Tca8113 and CAL-27 cells was explored by the CCK-8, EdU, and Transwell assay. Finally, the regulatory effect of miR-145-5p on its downstream gene KLF5 was evaluated. SNHG1 was abnormally upregulated in OSCC samples and linked to a poor prognosis of OSCC patients. Serving as an oncogene, SNHG1 strengthened proliferative and migratory functions of Tca8113 and CAL-27 cells. miR-145-5p was a key downstream target inducing the oncogenic role of SNHG1 in the OSCC process with KLF5 as its downstream gene. SNHG1/miR-145-5p/KLF1 axis is responsible for driving the malignant process of OSCC.
Highlights
Oral squamous cell carcinoma (OSCC) is the eighth most common cancer in the world, with more than 300,000 new cases each year [1]
A total of 31 pairs of OSCC and normal tissues were collected for detecting differential levels of SNHG1
SNHG1 was significantly upregulated in OSCC tissues (Figure 1(a))
Summary
Oral squamous cell carcinoma (OSCC) is the eighth most common cancer in the world, with more than 300,000 new cases each year [1]. Despite the great improvement made on therapeutic strategies, the 5-year survival of OSCC is lower than 50% mainly because of lymphatic metastasis [2]. Both radiotherapy and chemotherapy can be effective for OSCC patients. SNHG1 is capable of regulating miR-151-5p and EZH2, mediating colorectal carcinoma process [11]. E role of SNHG1 in the OSCC process is rarely reported. Our study identified an important feedback loop through the bioinformatics method and mainly explored its potential effect on regulating OSCC cell functions
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