Slower antibiotics degradation and higher resistance genes enrichment in plastisphere

Abstract

Microplastics (MPs) are increasingly entering the urban aquatic ecosystems, and the environmental significance and health risks of plastisphere, a special biofilm on MPs, have received widespread attention. In this study, MPs of polylactic acid (PLA) and polyvinyl chloride (PVC) and quartzite were incubated in an urban water environment, and the tetracycline (TC) degradation ability was compared. Approximatedly 24% of TC biodegraded in 28 d in the water-quartzite system, which is significantly higher than that in the water-PLA (17.3%) and water-PVC systems (16.7%). Re-incubation of microorganisms in biofilms affirmed that quartzite biofilm has a higher TC degradation capacity than the plastisphere. According to high-throughput sequencing of 16S rRNA and metagenomic analysis, quartzite biofilm contained more abundant potential TC degrading bacteria, genes related to TC degradation (eutG, aceE, and DLAT), and metabolic pathways related to TC degradation. An oligotrophic environment on the quartzite surface might lead to the higher metabolic capacity of quartzite biofilm for unconventional carbons, e.g., TC. It is also found that, compared with quartzite biofilm, the distinct microbes in the plastisphere carried more antibiotic resistance genes (ARGs). Higher affinity of MPs surface to antibiotics may lead to higher antibiotics stress on the plastisphere, which further amplify the carrying capacity for ARGs of microorganisms in the plastisphere. Compared to the nondegradable PVC MPs, surface of the biodegradable PLA plastics harbored significantly higher amounts of biomass and ARGs. Compared to the mineral particles, the capability of plastisphere has lower ability to degrade unconventional carbon sources such as the refractory organic pollutants, due to the abundance of carbon sources (adsorbed organic carbon and endogenous organic carbon) on the MPs surface. Meanwhile, the stronger adsorption capacity for pollutants also leads to higher pollutant stress (such as antibiotic stress) in plastisphere, which in turn affects the microbiological characteristics of the plastisphere itself, such as carrying more ARGs.