SLC2A3 variants in familial and sporadic congenital heart diseases in a Chinese Yunnan population.

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Solute carrier family 2member 3 (SLC2A3), is a member of a superfamily of transport protein genes. SLC2A3 played an important role in embryonic development. Previous research reported SLC2A3 duplication was reportedly associated with congenital syndromic heart defects. However, it is not clear whether the gene is associated with non-syndromic congenital heart disease. Our study aimed to elucidate the relationship between its variation and congenital heart disease. Genomic DNA extracted from the peripheral blood leukocytes of two families with CHD were sequenced with whole-exome sequencing to identify variations, used Sanger sequencing to investigate SLC2A3 variants in 494 Chinese patients with CHD and 576healthy unrelated individuals. In members from the two families, three with CHD had the SLC2A3 (rs3931701) C>T variant. Of the 494 patients with CHD, 394had gene variants (86had the TT type and 308had the CT type). Of the 576healthy controls, 272 participants had gene variants (36had the TT type and 236had the CT type). The TT type [p<0.0001, odds ratio (OR) =7.262, 95% confidence interval (CI) =4.631-11.388] and CT type (p<0.0001, OR =3.967, 95% CI =2.991-5.263) of SLC2A3 (rs3931701) significantly increased the risk of sporadic ASD in a Chinese Yunnan population. Single nucleotide variations of SLC2A3, particularly, the SLC2A3 (rs3931701) C>T variant increased the risk of CHD among the studied population.