Abstract
Size and shape distributions of nanoparticles can drastically contribute to the overall properties of nanoparticles, thereby influencing their interaction with different chemotherapeutic molecules, biological organisms and or materials and cell types. Therefore, to exploit the proper use of nanoparticles for various biomedical and biosensor applications, it is important to obtain well-separated monodispersed nanoparticles. However, gaining precise control over the morphological characteristics of nanoparticles during their synthesis is often a challenging task. Consequently, postsynthesis separation of nanoparticles is necessary. In the present study, demonstration on the successful onepot post-synthesis separation of anisotropic silver nanoparticles to near monodispersities using sucrose density gradient sedimentation. The separation of the nanoparticles was evidenced based on optical confirmation, and spectrophotometric and transmission electron microscopy measurements. Our results clearly demonstrate the facile separation of anisotropic silver nanoparticles using sucrose density gradient sedimentation and can enable the use of nanoparticles for various labeling, detection and biomedical applications.
Highlights
Nanoparticles (NPs) possess unique physico-chemical characteristics, which are dependent on their size and shape distributions [1,2,3,4,5]
Various separation techniques including chromatography, magnetic separation, electrophoresis, selective precipitation, membrane filtration, solvent or solution-based extractions and density gradient centrifugation have been used for isolating nanoparticles [5,13,14,15,16]
Anisotropic AgNPs with a wide range of size and shape distributions were synthesized using a previously reported bacterial-based biosynthesis method [20,23]. This technique results in a mixture of diverse particle sizes and shape distributions and illustrates the diverse NP mixtures that can result from biological and chemical based synthesis methods [11,20,21,22,23,24]
Summary
Nanoparticles (NPs) possess unique physico-chemical characteristics, which are dependent on their size and shape distributions [1,2,3,4,5]. Various separation techniques including chromatography, magnetic separation, electrophoresis, selective precipitation, membrane filtration, solvent or solution-based extractions and density gradient centrifugation have been used for isolating nanoparticles [5,13,14,15,16]. Density-based centrifugation is considered as a convenient method as it is economic, non-laborious, and does not involve the use of liquidsolid phase interactions and/or hazardous chemical reactions. This technique makes use of the sedimentation coefficients of nanoparticles in the surrounding medium, which can vary with nanoparticles form and mass [5], and has been successfully implemented to separate a wide range of nanoparticle using density gradient centrifugation rate [5,13]. Rapid separation of metal and quantum dot (CdSe) nanoparticles was demonstrated using non-hydroxylic organic as a density gradient [5]
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