Abstract
The elucidation of the biological function of protein complexes is an area of intense research efforts but isolation and analysis of the complexes is challenging as they can be very large and unstable. Superose™ 6 Increase is a new agarose‐based SEC (size exclusion chromatography) medium with a wide separation range, Mr 5000 to 5 000 000 for globular proteins. The wide separation range allows separation of very large proteins, complexes and membrane proteins both for preparative and analytical purposes under mild, non‐dissociative conditions.Separation of a protein involved in structural coloration on Superdex 200 10/300 resulted in co‐elution of two differently sized protein populations. The wider separation range of Superose 6 Increase 10/300 enabled separation of the two populations. Based on elution position, the size of the separated material was estimated to Mr > 700 000Purification of membrane proteins often require addition of expensive detergents in the buffer to keep the proteins stable and thus low consumption of buffer is important. Purification of a membrane protein complex on Superose 6 Increase 5/150 (3 ml bed volume) resulted in good separation from aggregated material and degradation products.
Published Version
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