Abstract
Size exclusion chromatography (SEC) hyphenated to inductively coupled plasma – mass spectrometry (ICP-MS), as a specific detector for metals, has been used for monitoring and determining metal-low molecular weight organic compound (LMWC) complexes in natural wines. SEC with UV detection (wavelength of 205 nm) was used for monitoring organic compounds eluted from the chromatographic column. SEC-UV has revealed the presence of low molecular weight compounds (commonly three fractions of molecular weights ranging from 230 to 1579 Da).’ Further experiments using ICP-MS as a detector showed that elements such as B, Cu, Li, Mn, Ni, Ti, and Zn are bound to compounds of molecular weights within the 338–1579 Da range. Total metal concentrations, as well as metal concentrations in SEC fractions were also assessed in several monovarietal red (five varieties) and monovarietal white (three varieties) wines.
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