Abstract
Empty viral protein shells or viral antigen have been identified in the nuclei of Chinese cabbage infected with turnip yellow mosaic virus, Nicotiana glutinosa infected with eggplant mosaic virus, cucumber infected with okra mosaic virus, and bean infected with Desmodium yellow mottle virus. For turnip yellow mosaic virus, viral coat protein can be detected from an early stage of infection at two sites: (i) in the nucleus, where coat protein in the form of empty protein shells accumulates, and (ii) in the electron-lucent zones in the cytoplasm over the clustered vesicles of chloroplasts. We conclude that this electron-lucent material contains pentamers and hexamers of coat protein because particles seen within it on freeze-fracturing have the right dimensions, and because the material reacts with antibody directed against intact TYMV. A mechanism is proposed by which turnip yellow mosaic virus particles are assembled at the necks of the peripheral vesicles from an emerging strand of RNA combining with pentamers and hexamers of coat protein lying in an oriented fashion within the lipid bilayer of the outer chloroplast membrane.
Published Version
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