Abstract
The distribution and translocation as well as the biosynthetic sites of 3-isobutyl-2-methoxypyrazine (IBMP) in bell pepper plants (Capsicum annuum) were examined. For IBMP-quantification, a stable-isotope dilution assay (SIDA) was developed, using headspace solid-phase microextraction (HS-SPME) combined with gas chromatography and time-of-flight mass spectrometry (ToF-MS). While IBMP was the most abundant MP in all aerial plant parts, IPMP was dominating in root tissues. The highest IBMP-levels were found in unripe bell pepper fruits. Moreover, feeding experiments with [2H10]-l-leucine revealed that IBMP is biosynthesized in all plant parts, while in roots and ripe bell pepper fruit tissues no incorporation of the labelled precursor was detectable. A potential phloem translocation of IBMP could not be detected.
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