Abstract
The systematic replacement of nucleotides in DNA fragments isolated by recombinant DNA technology can be achieved by a variety of chemical and enzymatic manipulations. Application of these procedures to DNA that encodes the amino acid sequence of a protein makes it possible to obtain specifically mutated protein when the encoding DNA is expressed in living cells. This variant of genetic engineering — protein engineering by site-directed mutagenesis — is having a major impact on protein structure—function analysis and in the production of proteins with changed or novel properties.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
