Abstract
Copper uptake potential of different bacterial strains isolatedfrom contaminated soil was compared. The bacterial strainidentified as Bacillus polymyxa showed maximum copperuptake capacity. An attempt was made to elucidate the site ofinteraction of Cu(II) with B. polymyxa using electronparamagnetic resonance (EPR) studies. The EPR spectra showedthat accumulated copper formed complexes with oxygen andnitrogen which may be the carboxyl groups of cell-wallpeptidoglycan and nitrogen atoms of aminosugars or structuralproteins. The absence of super hyperfine splitting in theg ⊥ region of EPR spectra of treated B. polymyxa cellsafter Cu(II) uptake indicated that copper could be coordinatedwith the oxygen atoms from carboxyl groups of peptidoglycan inthe cell walls.
Published Version
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