Abstract
l -Cysteine can enhance the fluorescence of SiQDs/Cu 2 -β-CD nanoclusters at pH 8; uric acid can attenuate their fluorescence at pH 13. SiQDs = silicon quantum dots ; Cu 2 -β-CD = Cu-modified- β -cyclodextrin; l -Cys = l -cysteine. • SiQDs/Cu 2 -β-CD nanoclusters were prepared via coordination-driven self-assembly. • N anoclusters had distinct fluorescence responses for l -cysteine and uric acid. • N anoclusters can achieve the mutual non-interference detection of uric acid and l -cysteine. The fluorescence probes based on quantum dots (QDs) have been widely used in the detection of uric acid (UA), but other biomolecules such as biothiols, ascorbic acid and dopamine have interference with the detection result due to their similar chemical properties. Herein, SiQDs/Cu 2 -β-CD nanoclusters were prepared via coordination-driven self-assembly between Cu 2 -β-CD and –NH 2 on the surface of SiQDs , and UA could attenuate the fluorescence of the nanoclusters under strong alkali conditions, while l -cysteine ( l -Cys) had no effect. Under the optimum conditions, the decreased fluorescence intensity is linearly proportional to the concentration of UA in the range of 0.025–0.15 mM with a detection limit of 4.9*10 -3 mM. Furthermore, under neutral or weak base conditions, l -Cys could improve the fluorescence of the nanocluster, but UA had no effect, which could also be used to detect l -Cys. This work provides a sensitive, selective and convenient fluorescence method for UA and l -Cys detection in the present of another interfering biomolecule.
Published Version
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