Single‐Walled Carbon Nanotubes/Au–Mesoporous Silica Janus Nanoparticles as Building Blocks for the Preparation of a Bienzyme Biosensor

Abstract

AbstractGold–mesoporous silica Janus nanoparticles were prepared through a masking strategy and further employed as building blocks for the preparation of bienzyme biosensors through toposelective immobilization of the corresponding enzymes. The system was evaluated by immobilizing glucose oxidase and horseradish peroxidase on the Au and mesoporous silica faces, respectively. The anisotropic nanoparticles were deposited on glassy carbon electrodes coated with single‐walled carbon nanotubes in order to construct an amperometric biosensor for glucose detection. The enzymatic electrode showed excellent electroanalytical performance with a fast response in about 6 s, a linear range of 490 nm–600 μm, a high sensitivity of 4.3 mA M−1 , and a low detection limit of 360 nm for glucose. This biosensor was successfully employed to determine the content of this monosaccharide in commercial soft drink samples.