Single-sweep volumetric optoacoustic tomography of whole mice

Abstract

Applicability of optoacoustic imaging in biology and medicine is determined by several key performance characteristics. In particular, an inherent trade-off exists between the acquired field-of-view (FOV) and temporal resolution of the measurements, which may hinder studies looking at rapid biodynamics at the whole-body level. Here, we report on a single-sweep volumetric optoacoustic tomography (sSVOT) system that attains whole body three-dimensional mouse scans within 1.8 s with better than 200 μm spatial resolution. sSVOT employs a spherical matrix array transducer in combination with multibeam illumination, the latter playing a critical role in maximizing the effective FOV and imaging speed performance. The system further takes advantage of the spatial response of the individual ultrasound detection elements to mitigate common image artifacts related to limited-view tomographic geometry, thus enabling rapid acquisitions without compromising image quality and contrast. We compare performance metrics to the previously reported whole-body mouse imaging implementations and alternative image compounding and reconstruction strategies. It is anticipated that sSVOT will open new venues for studying large-scale biodynamics, such as accumulation and clearance of molecular agents and drugs across multiple organs, circulation of cells, and functional responses to stimuli.