Abstract
The present report describes a single-step method for derivatization of dopamine and several structurally related compounds, either a catecholic or a monophenolic amine, acid, alcohol or glycol present in aqueous solutions. Also, nanogram levels of these compounds may be assayed by gas chromatography with electron-capture detection following derivatization. For the determination of optimum reaction conditions, aqueous solutions of either [ 14C] dopamine or [ 3H] norepinephrine were reacted with th derivatization agent, heptafluorobutyryl chloride. A mass spectrum of the derivative of dopamine confirmed the formation of triheptafluorobutyryl dopamine. To determine the sensitivity and specificity of the derivatization method, a number of biological samples from rats and humans were analyzed for dopamine and 3,4-dihydroxyphenylacetic acid. The urinary analyses showed that conjugation may be the major metabolic pathway for dopamine and 3,4-dihydroxyphenylacetic acid in rats as well as in humans. The present method should prove convenient to determine the urinary sulfate conjugate of 3,4-dihydroxyphenylacetic acid, a non-invasive indicator of central nervous system dopaminergic activity, and other catecholamine metabolites of clinical interest.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
