Abstract
We present a single-shot isotropic quantitative phase imaging method based on color-multiplexed differential phase contrast. In our method, the illumination source is modulated by an LCD into an annular color-multiplexed pattern matching the numerical aperture of the objective precisely to maximize the frequency response for both low and high frequencies (from 0 to 2NAobjλ). In addition, we propose an alternating illumination scheme to provide a perfectly circularly symmetrical phase transfer function, achieving isotropic imaging resolution and signal-to-noise ratio. A color camera records the light transmitted through the specimen, and three monochromatic intensity images at each color channel are then separated and utilized to recover the phase of the specimen. We display the derivation, implementation, simulation and experimental results, which demonstrate that our method accomplishes high resolution, noise-robustness and reconstruction accuracy phase reconstruction at camera-limited frame rates.
Highlights
We propose an alternating illumination scheme to provide a perfectly circularly symmetrical phase transfer function, achieving isotropic imaging resolution and signalto-noise ratio
We present a single-shot isotropic quantitative phase imaging method based on color-multiplexed differential phase contrast
In contrast to qualitative imaging methods, Quantitative phase imaging (QPI) approaches, such as holography,6 transportof-intensity equation (TIE),7,8 differential phase contrast (DPC),9,10 and Fourier ptychographic microscopy (FPM),11,12 recover the phase of the sample from intensity variation caused by optical pathlength change, allowing for various quantitative studies, such as the measurement of cellular mechanical properties and dynamic transport of intracellular structures
Summary
Yao Fan,1,2,3,a) Jiasong Sun, Qian Chen,1,2,b) Xiangpeng Pan, Maciej Trusiak, and Chao Zuo1,2,3,c)
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