Single-Shot Common-Path Off-Axis Dual-Wavelength Digital Holographic Microscopy Based on Two-Dimensional Grating Diffraction

Jieyu Wang,Huaying Wang,Zhao Dong,Qiaofen Zhu,Yafei Gao,Wenjian Wang,Xue Wang,Gaofu Men

doi:10.3389/fphy.2022.822714

Abstract

We present a single-shot dual-wavelength common-path off-axis digital holographic microscopic (CO-DHM) imaging method based on two-dimensional grating diffraction. This method improves the utilization rate of the interference field under the limited photosensitive size of the camera, and further expands the original camera’s field of view (FOV). In addition, the mode of orthogonal carrier frequencies close to the diagonal direction can optimize the utilization of the camera’s spatial bandwidth. Compared with the traditional dual-wavelength CO-DHM using one-dimensional grating or prism beam splitting, this method effectively avoids the aliasing of high-frequency components of the +1-order spectrum of different wavelengths in the frequency domain. We provide quantitative phase imaging experiments for the full FOV of USAF resolution chart, onion epidermal cells and standard polystyrene beads. The results prove that the system can enlarge the interferometric FOV by nearly 74.0% without changing the imaging parameters, such as magnification and resolution, and can achieve high-precision quantitative phase imaging with only a single hologram.

Highlights

Digital holographic microscopy (DHM) combines traditional optical microscopy imaging technology and holographic imaging technology to quantitatively obtain the amplitude and phase distribution of the original object light in the field of microscopic imaging [1,2,3,4,5,6]
We present a single-shot dual-wavelength common-path off-axis digital holographic microscopic (CO-DHM) imaging method based on two-dimensional grating diffraction
The results prove that the system can enlarge the interferometric field of view (FOV) by nearly 74.0% without changing the imaging parameters, such as magnification and resolution, and can achieve high-precision quantitative phase imaging with only a single hologram

Summary

INTRODUCTION

Digital holographic microscopy (DHM) combines traditional optical microscopy imaging technology and holographic imaging technology to quantitatively obtain the amplitude and phase distribution of the original object light in the field of microscopic imaging [1,2,3,4,5,6]. If the selected area is too small during frequency domain filtering, High frequency components will be lost To solve this problem, MIT Poorya Hosseini and others developed a dual-wavelength interference microscope in 2018, which provides single-lens off-axis interferometry by encoding two spectral images at orthogonal spatial frequencies, but the optical path uses two 4f systems [26]. One of them is the spatial phase scanning digital holographic imaging technology, which scans the sample on a reference light field, periodically obtains multiple frames of sample phase images, and uses algorithms to combine them into a large field of view holographic image This type of method sacrifices the time resolution or the range of the dynamic area allowed in the recorded image [29]. To prove the high precision of the system, the large field of view quantitative phase imaging capability, the imaging results of the resolution plate, standard polystyrene beads and onion epidermal cell are provided

EXPERIMENTS AND METHODS

CONCLUSION

DATA AVAILABILITY STATEMENT