Abstract
Cytoplasm regions of crayfish stretch receptor neurons were irradiated by the microbeam of a pulse-periodic dye laser (diameter 5 μm, pulse duration 15 nsec, pulse frequency 400 Hz). Irradiation wavelengths varied from 434 to 600 nm, and beam intensity (at 441.6 nm) from 0.25 to 5 × 10 3 W cm −2 (mean), or from 0.4 to 8 × 10 8 W cm −2 (peak). Under irradiation neuron firing typically accelerated and then inhibited. In some cases it accelerated again and then abruptly and irreversibly ceased. Action spectra of parameters describing different response phases had the flavin-like maximum at 460 nm. This suggests flavins as a primary intracellular photoreceptor for blue laser light. It was also found that firing acceleration rate quadratically depended on peak beam intensity. This finding indicates the participation of two-photon processes in neuronal membrane alteration leading to firing acceleration. Parameters describing inhibition phase depended on beam intensity linearly. That is characteristic for usual single-photon process.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of Photochemistry & Photobiology, B: Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.