Abstract
Staphylococcus aureus is gram-positive bacteria that often infect the skin. S. aureus has been experiencing resistance to several antibiotics. One of the solutions to overcome the resistance is by using garlic that is believed by the society can overcome bacterial infection. The study aimed to find out the influence of the variation in concentration of local single clove garlic essential oil used on the inhibition and damage of morphological structure of S. aureus bacteria. The inhibition test was conducted using disc diffusion method. The experiment groups consisted of 1% DMSO as negative control, vancomycin 30µg/ml as positive control and single clove garlic essential oil (25 mg/ml, 50 mg/ml, 75 mg/ml and100 mg/ml) as treatment groups. The diameter of inhibition zone was measured using calipers. The morphological damage of the bacterial cells can be seen using Scanning Electron Microscopy (SEM) with magnification of 25000x. The result of Kruskal-Wallis test analysis indicated that the extract of local garlic essential oil has inhibitory activities against S. aureus bacteria (P<0.05). The damage to the morphological structure of bacterial cells with the administration of 30 µg/ml vancomycin was equal to 100 mg/ml single clove garlic essential oil extract. Single clove garlic essential oil can be used as an alternative treatment for skin infection diseases by inhibiting S. aureus growth.
Highlights
INTRODUCTIONBacteria infecting the skin are usually from Staphylococcus aureus species
Skin infection disease caused by bacteria is a health problem among the societies
Active compounds contained in single clove garlic which has antibacterial activity for example are allicin, ajoene, diallyl sulfide (DAS), diallyl disulfide (DADS) dan diallyl trisulfide (DATS)
Summary
Bacteria infecting the skin are usually from Staphylococcus aureus species. The active compounds in single clove garlic relatively high compared to regular garlic caused by all substances were gathered in one clove. Active compounds contained in single clove garlic which has antibacterial activity for example are allicin, ajoene, diallyl sulfide (DAS), diallyl disulfide (DADS) dan diallyl trisulfide (DATS). Allicin and ajoene have ability as an antibacterial by totally inhibit the RNA synthesis and partially inhibit the DNA and protein synthesis This mechanism causes inhibition in bacterial growth that lead to the death of the bacterial cells (Eltaweel, 2014; Rehman & Mairaj, 2013). This study was intended to find out the effect of administration as well as concentration variation used of essential oil from single clove garlic (Allium sativum L.) to the inhibition and damage to cell structure of S. aureus bacteria
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