Abstract
Conventional wisdom suggests that viable human embryos are the result of faithful cloning of euploid zygotes. However, study of blastocysts grown in vitro often identifies mosaicism that is likely the result of post-fertilization mitotic errors1. Yet, the true incidence of embryonic mosaicism remains poorly understood. Although prior work has studied cleavage stage embryos or blastocyst biopsies, to our knowledge, whole embryonic analysis via single cell sequencing of every cell from a human blastocyst has yet to be performed.
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