Abstract
In rheumatological studies, visualization of Ca2+ dynamics in intact cells as direct experimental evidence of Ca2+-dependent signal pathways is generally used to monitor the function of immune cells at first glance. Ability to monitor Ca2+ signaling in living cells would greatly facilitate advances in the functional dissection of immune cells. In this chapter, we describe a basic technique and methods of data analysis for single-cell real-time Ca2+ monitoring using Fluo-4 labeling, which is a single-wavelength Ca2+ indicator.
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