Single-atom Ru loaded on layered double hydroxide catalyzes peroxymonosulfate for effective E. coli inactivation via a non-radical pathway: Efficiency and mechanism

Abstract

The Fenton-like processes are considered to be one of the most promising strategies for inactivating bacteria due to their capacity to produce reactive oxygen species (ROS). Herein, a catalytic system for efficient inactivation of Escherichia coli (E. coli) was developed by anchoring single-atom Ru on layered double hydroxides (LDH). The Ru/NiFe-LDH catalyst showed excellent performance in activating peroxymonosulfate (PMS) to inactivate E. coli. Under the combined action of the ultra-low concentrations of Ru/NiFe-LDH (40 mg/L) and PMS (5 mg/L), 7 log E. coli can be totally inactivated within 90 s. This was attributed to the combined effect of single-atom Ru adsorption to E. coli and the ROS produced in situ. Mechanism studies indicated that the 1O2 with electrophilic properties was the key active species responsible for the rapid inactivation of E. coli. The E. coli inactivation process suggested that the ROS produced first attacked the outer membrane of the cell, then the antioxidant enzymes in the cell were induced, the macromolecule substances were released and mineralized, eventually leading to irreversible cell death. This work firstly loads monoatomic Ru on LDH for bacterial inactivation, providing a feasible method for rapid inactivation of E. coli.