SIMULTANEOUS SEPARATION OF GALLIC ACID, PROTOCATECHUIC ACID, RUTIN, MANGIFERIN AND CHROMATOGRAPHIC PROFILING OF SOME INDIAN MEDICINAL PLANTS

Abstract

Background: Plants and plant-based extracts have been used across the globe for management of various ailments and disorders. The biological and pharmacological activities of plants and their extracts are primarily attributed to polyphenols, avonoids, and other secondary metabolites. High Pressure Liquid Chromatography (HPLC) is a reliable, sensitive, and quick method to identify bioactive ingredients in complex plant extracts. In the present study, we have developed a quick and sensitive HPLC method for separation of four polyphenols, namely, gallicMethods: acid, protocatechuic acid, rutin, and mangiferin. Further, we used the developed HPLC method for the analysis of plant extracts obtained from Asparagus racemosus, Azadirachta indica, Capsicum annuum, Nigella Sativa, Phyllanthus niruri, Rosmarinus ofcinalis, Rheum palmatum, Withania somnifera, Withania somnifera, and Zingiber ofcinale. The HPLC method developed in the present study was highly effectiveResults: in separating gallic acid, protocatechuic acid, rutin, mangiferin. The method also provides excellent phytoconstituent ngerprinting of complex plant extracts of medicinal plants. The gradient mode HPLC method developed in the present study is highly useful for identicationConclusion: of plant polyphenols in complex plant-based extracts and generating HPLC chromatograms for complex plant extracts. The present method, thus, can be used for phytoconstituent proling, quality control of herbal drugs, and assessing adulteration of plant extract or nutraceuticals.