Simultaneous selenite reduction and nitrogen removal using Paracoccus sp.: Reactor performance, microbial community, and mechanism

Abstract

Selenium-containing wastewater has a high concentration of nitrogen compounds (ammonia nitrogen [NH4+-N]), leading to water pollution. Thus, the simultaneous reduction of selenium and removal of nitrogen compounds during wastewater treatment has become the top priority. However, the exogenous bacteria that can simultaneously reduce selenite and remove ammonia nitrogen and colonize in the wastewater treatment systems have not been reported. Additionally, the effects and the underlying mechanism of biofortification on the reduction and removal efficiency of the microorganisms remain unclear. In this study, we investigated the simultaneous selenite reduction and nitrogen removal efficiency of Paracoccus sp. (strain SSJ) isolated from selenium-contaminated soil and explored biofortification effects on the composition and structure of the microbial community. Using sequencing biofilm batch reactors (SBBRs), the structural and functional characteristics of the microbial community were systematically compared between the control (group A) and biofortified (group B) groups. Strain SSJ could simultaneously reduce 63.28% of selenite and remove 93.05% of NH4+-N within 24 h. Moreover, no accumulation of nitrate nitrogen (NO3−-N) and nitrite nitrogen (NO2−-N) was observed in the reaction process. The performance and stability of the SBBRs enhanced by strain SSJ were greatly improved. Illumina sequencing results showed that strain SSJ was surprisingly colonized, and Paracoccus was the predominant genus in group B (relative abundance: 13.93%). Moreover, PICRUSt2 analysis results suggested that the microbial community in group B demonstrated increased rates of ammonia nitrogen removal through ammonia assimilation and selenite reduction through sulfur metabolism and glutathione-mediated selenite reduction pathway. In summary, our findings shed light on the mechanism for simultaneous selenite reduction and nitrogen removal by biofortification and provide novel microbial resources for the treatment of selenite-containing wastewater.