Simultaneous removal of As(III) and Cu(II) from real bottom ash leachates by manganese-oxidizing aerobic granular sludge: Performance and mechanisms

Abstract

Manganese-oxidizing aerobic granular sludge (Mn-AGS) is a novel extension of AGS technology to treat arsenic (As) in organic wastewater. In this study, Mn-AGS was first applied to treat real wastewater (bottom ash leachates) containing high levels of As(III) and Cu(II) in a sequencing batch reactor (SBR) for 91 days. Influent and effluent As(III), As(V), Cu(II), as well as pH and chemical oxygen demand (COD) were monitored daily, and sludge was collected regularly for morphological observation, chemical characterization, and microbial analysis. The results indicated that As(III) and Cu(II) could be efficiently removed from wastewater (∼83% and ∼100%, respectively), but the performance was sensitive to pH variation, especially for As(III). The removed As and Cu were mostly bound to carbonates (60.2 ± 2.0% and 70.0 ± 0.6%, respectively) and Fe/Mn oxides (28.2 ± 1.6% and 14.6 ± 0.5%, respectively) in the final sludge. Influent As(III) was partially oxidized into As(V), and high fractions of As(V) were obtained in the Fe/Mn oxide-bound phase. Unexpectedly, microbial analysis revealed that community richness was only slightly changed when the influent was acidized (pH 4.0) but greatly reduced after the influent pH back to 6.0. It could be explained by that acid-fast bacteria rapidly grew after pH recovery and eliminated non-acid-fast bacteria. This work further supported the practical application of Mn-AGS to treat As(III)-containing organic wastewaters.