Abstract
A simple and rapid high performance liquid chromatography coupled with diode array detector (HPLC-DAD) method was developed for simultaneous quantification of three chemical types of bioactive compounds including an alkaloid (epigoitrin), six nucleosides (uracil, cytidine, hypoxanthine, uridine, guanosine and adenosine), and four phenylpropanoids (syringin, clemastanin B, indigoticoside A and isolariciresinol) in Radix Isatidis and its relevant pharmaceutical dosage forms. The developed method was successfully applied to analyze 32 batches of samples with good linearity (R2, 0.9966-0.9999), precisions (relative standard deviation (RSD), 0.24-4.61%), repeatability (RSD, 0.39-4.93%), stability (RSD, 1.18-4.29%) and recovery (range, 95.4-104.6%) of the eleven compounds. The limits of detection (LOD) and limits of quantification (LOQ) of the method ranged 0.0383-0.1744 and 0.1550-0.5467 µg mL-1, respectively. The results indicated that the newly established HPLC-DAD method was sensitive, precise, accurate and reproducible. It could be applied to the quality control of Radix Isatidis and its relevant pharmaceutical dosage forms.
Highlights
The well-known traditional Chinese medicine Radix Isatidis is the dried root of the plant Isatis indigotica Fort
Cytidine, hypoxanthine, uridine, guanosine and adenosine are the main nucleosides in Radix Isatidis.[5]
A simple and reliable high performance liquid chromatography (HPLC) coupled with diode array detector (DAD) method has been developed for simultaneous determination of these eleven main bioactive compounds (Figure 1) in Radix Isatidis and its pharmaceutical dosage forms
Summary
The well-known traditional Chinese medicine Radix Isatidis (common name: Banlangen) is the dried root of the plant Isatis indigotica Fort. In order to control the quality of the herbs and its pharmaceutical dosage forms from different regions or manufacturers, it is very necessary to develop a simple and effective HPLC method for simultaneously quantifying these three different chemical types of bioactive compounds.
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