Abstract
RNA-dependent DNA polymerase and gs-antigen were purified simultaneously from Rauscher leukemia virus by sequential column chromatography on phosphocellulose. The partially purified RNA-dependent DNA polymerase has a molecular weight of 70,000 and is free of cellular DNA polymerase, deoxynucleotidyl terminal transferase, RNase and DNase. The partially purified RNA-dependent DNA polymerase can efficiently copy oligo dT·poly rA and oligo dG·poly rC. The purified gs-antigen shows a single band on SDS-polyacrylamide gel with a molecular weight of 30,000. It is active immunologically and possesses both group and interspecies activity.
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