Abstract
We selected the DYS19, DYS385, DYS389, DYS390, DYS392, DYS393, DYS483, and amelogenin loci, and designed a new pair of primers to minimize the fragment sizes of these loci as much as possible. As a consequence, these loci were able to detect in the range of 79–259 bp using multiplex PCR amplification. The optimum DNA amount was 100 pg to 10 ng. The haplotype diversity was 0.9979.
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