Abstract
We have developed an easily operable scanning near-field optical microscope for multicolor fluorescence imaging by applying a highly sensitive charge-coupled-device (CCD) camera. The CCD camera is used as a variable area photosensor, and a mean intensity of a selected area in the CCD camera is converted to a numerical value. By combining a spectrometer with the CCD camera, arbitrary wavelength ranges can be selected to provide plural fluorescence images separated by fluorescence wavelength. The function of the system is demonstrated by imaging mixed fluorescence particles and a two-color stained chromosome sample of Hordeum vulgare treated with a two-color fluorescent banding stain method.
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