Abstract

Abstract Different ways for studying DNA synthesis and DNA methylation were investigated. On the one hand the reactions were measured by incorporation of several specific precursors, taking into ac­ count that pool alterations might influence the results. The best way to avoid this is the direct conversion of DNA cytosine to DNA 5-methyl-cytosine by using [14C]-cytidine. According to our results we propose to calculate the correct DNA methylation rate in term of the specific radio­ activity of the separated DNA bases

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call