Abstract
This paper proposes the design and implementation of a micro-electrode array (MEA) for neuroblastoma cell culturing. It also explains the implementation of a multi-photon microscope (MPM) customized for neuroblastoma cell excitation and imaging under ambient light. Electrical signal and fluorescence images were simultaneously acquired from the neuroblastoma cells on the MEA. MPM calcium images of the cultured neuroblastoma cell on the MEA are presented and also the neural activity was acquired through the MEA recording. A calcium green-1 (CG-1) dextran conjugate of 10,000 D molecular weight was used in this experiment for calcium imaging. This study also evaluated the calcium oscillations and neural spike recording of neuroblastoma cells in an epileptic condition. Based on our observation of neural spikes in neuroblastoma cells with our proposed imaging modality, we report that neuroblastoma cells can be an important model for epileptic activity studies.
Highlights
In recent times, neuroscience has received much attention as important research field for understanding the connections between neuron cells and reaction mechanisms
Our study focused on a simultaneous measurement system using a micro-electrode array of simple structure, and on recording neural spikes from cultured neuroblastoma cells under epileptic conditions
The neuroblastoma cells were cultured on the micro-electrode array (MEA) for 7 days allowing the cells to attach to the MEA surface
Summary
Neuroscience has received much attention as important research field for understanding the connections between neuron cells and reaction mechanisms. It became possible to measure reaction signals from tens to hundreds of neurons [6], but in all this research, there exists a limitation that the neurons cannot be observed while acquiring signals from them. To overcome this limitation, studies using two-photon or multi-photon microscopy as imaging tools have been underway since the late 1990s [7,8,9]. Such non-linear microscopies are becoming standard tools for defining molecular mechanisms in the field of cell-based engineering and bio-medical research
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