Simultaneous HPLC Quantitative Analysis of Nine Bioactive Constituents in Scirpus Yagara Ohwi. (Cyperaceae).

Abstract

The tuber of Scirpus yagara Ohwi. (Cyperaceae) has long been used in traditional Chinese medicine (TCM). Several chemical constituents isolated from it possess a variety of physiologically activities such as anti-inflammatory, antitumor and antioxidant. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of nine active components in tubers and aerial parts of S. yagara. The analysis was performed on a YMC-Pack ODS-A column (4.6 × 250 mm, 5 μm, 30 nm) with a multilinear gradient mobile phase of water-formic acid (100 : 0.2, v/v) and methanol. The established HPLC method was validated in terms of linearity, sensitivity, precision, accuracy, recovery and stability. All analyzed components were detected in the whole tested samples, and the contents of most components in the aerial parts were even higher than those in the tubers. Moreover, the best harvest period was discovered to be November, which is different from the traditional. The method developed was successfully applied for simultaneous qualitative and quantitative analysis of nine active components in S. yagara.