Simultaneous HPLC Quantification of Four Lignan Glycosides in Sesame Seeds with the Single Reference Standard Method

Abstract

Seven lignans including four lignan glycosides and three lignans were isolated from 70% methanolic extract of defatted sesame seeds. The chemical structures of the isolated compounds were identified by UV, 1H and 13C NMR, and HR-MS spectra. To overcome the difficulty and high cost for the preparation of lignans, the single reference standard method for the simultaneous determination of multi-components (SSDMC) with high-performance liquid chromatography (HPLC) was developed for simultaneous quantification of four lignin glycosides in sesame seeds. All four lignin glycosides had good linear concentration-measurement relationships (r2 ≥ 0.9992). The relative deviation of the method was less than 4.9% for intra- and inter-day assays and the average recovery varied from 93.3 to 101.2%. The method was also successfully applied to determine four lignan glycosides in eighteen white sesame seeds and ten black sesame seeds. The results demonstrated that the single reference standard method could be used for quality control of sesame seeds and related products since the reference standards are not commercially available.