Simultaneous HPLC Determination of Arbutin, Niacinamide and 3-O-Ethyl Ascorbic Acid in Whitening Cream Products in the Presence of Parabens.

Abstract

Arbutin, niacinamide and 3-O-ethyl ascorbic acid (a new generation of vitamin C derivatives) are compounds that have a whitening effect on skin and are widely used in whitening cream products wherein parabens such as methyl paraben, ethyl paraben, propyl paraben and butyl paraben are also often added as preservatives. This study aims to develop a validated high-performance liquid chromatography (HPLC) method that can be used to determine arbutin, niacinamide and 3-O-ethyl ascorbic acid simultaneously in whitening cream products without interference from the parabens. The optimum conditions for the HPLC system were obtained using ODS-3 RP-C18 Inertsil column, mobile phase consisting of a mixture of aquabides, methanol and acetonitrile with gradient elution mode. Detection was carried out using a UV detector at 220nm. Validation studies demonstrated a good linearity for all analytes over each range concentration with a correlation coefficient >0.999 and Vx0 < 2%. The accuracy test also met the requirements with the recoveries being 96.93-99.55%, 98.60-99.73% and 97.88-100.63% for arbutin, niacinamide and 3-O-ethyl ascorbic acid, respectively. Intra-day and inter-day precision test gave a relative standard deviation (% RSD) of <2% along with a HorRat value of <2 for all analytes. The results of this study indicate that the developed HPLC method has a good selectivity, linearity, accuracy and precision. Due to its simplicity, the method can be used to analyze arbutin, niacinamide and 3-O-ethyl ascorbic acid in the presence of parabens in whitening cream products simultaneously.