Simultaneous Fourth Order Derivative Spectrophotometric Determination of Cobalt, Nickel and Copper Using Anthrone Phenylhydrazone (APH)

Abstract

The reagent anthrone phenylhydrazone (APH) is used for the simultaneous determination of Co(II), Ni(II) & Cu(II) by fourth order derivative spectrophotometric method. The reagent APH gives light yellow colour with Co(II), Ni(II) & Cu(II) solution in basic medium. The maximum peaks were observed between 426.3-625 nm for Co(II), Ni(II) & Cu(II) in basic buffer solution of pH 10. The molar absorptivity and Sandells sensitivity of Co(II), Ni(II) & Cu(II)-APH complexes are computed, the molar absorptivity are 1.5 x10 4 L/mol/cm, 2.0 x10 4 L/mol/cm and 2.163 x10 4 L/mol/cm respectively. The Sandells sensitivity are 0.0066 µg/cm 2 , 0.005 µg/cm 2 and 0.0026 µg/cm 2 respectively. The stability constant of Co(II), Ni(II) & Cu(II)-APH complexes are 1.530 x10 5 , 2.486 x10 4 and 2.96 x10 4 respectively. The effect of various concentrations of Co(II), Ni(II) & Cu(II) ions on amplitude also studied. The samples of grape leaves, sesame, laver, mung bean and alloy samples were analyzed by the proposed method. The results of the food samples and alloy samples are good agreement with the results of APARI, AAS methods.