Simultaneous estimation of tramadol and piroxicam by UV spectrophotometer and RP-HPLC

Abstract

Several clinical studies have shown that the use of a combination of Tramadol and Piroxicam has displayed a better analgesic effect than the individual drug with the occurrence of fewer side effects. As such, there is no analytical method available for Qualitative and Quantitative determination of particular drugs in both combinations. Different analytical techniques can be applied for multicomponent analysis, including; spectrophotometer, chromatography, and electrophoresis. In the present work, the analytical methods of qualitative and quantitative analysis for combinations of Tramadol and Piroxicam have been used employing UV spectrophotometer and HPLC. Two simple, accurate, rapid, and selective analytical procedures were developed and validated for the simultaneous estimation of Tramadol HCl and Piroxicam using UV spectrophotometer and High-performance liquid chromatography with UV detection (HPLC-UV). The absorption Subtraction technique was utilized in the Simultaneous determination of the UV spectrophotometer. The HPLC method employs a C18 Phenomenex column (250 × 4.6, 5 µm), 20 µL injection volume, column temperature controlled at 30° C, detection at 282 nm, Trifluoroacetic acid-pure acetonitrile (70: 30 v/v) mobile phase pumped at the rate. Parameters consisting of linearity, precision, accuracy, robustness, ruggedness, detection and quantitation are studied. From the investigations, the Absorptive factor of Tramadol HCl and Piroxicam at both the isosbestic points has been found. The percentage assay of 108.73 ± 1.61 % and 104.54 ± 0.94 % for Tramadol HCl and Piroxicam. The objective of validation of analytical procedures is to demonstrate that it is suitable for its intended purpose.