Simultaneous enzymatic hydrolysis and anaerobic biodegradation of lipid-rich wastewater from poultry industry

Gisanara Dors,Ernandes B Pereira,Heizir F De Castro,Agenor Furigo,Adriano A Mendes

doi:10.1007/s13201-012-0075-9

Abstract

Simultaneous enzymatic hydrolysis and anaerobic biodegradation of lipid-rich wastewater from poultry industry with porcine pancreatic lipase at different concentrations (from 1.0 to 3.0 g L−1) were performed. The efficiency of the enzymatic pretreatment was measured by the Chemical Oxygen Demand (COD) removal and formation of methane. All samples pretreated with lipase showed a positive effect on the COD removal and formation of methane. After 30 days of anaerobic biodegradation the methane production varied from 569 ± 95 to 1,101 ± 10 mL for crude wastewater and pretreated at 3.0 g L−1 enzyme, respectively. COD removal of wastewater supplemented at different enzyme concentrations was found to be threefold higher than crude wastewater. The use of lipases seems to be a promising alternative for treating lipid-rich wastewaters such as those from the poultry industry.

Highlights

Chemical Oxygen Demand (COD) removal of wastewater supplemented at different enzyme concentrations was found to be threefold higher than crude wastewater
The results show that the supplementation of lipases at low concentration was enough to improve the assimilation of organic compounds through anaerobic biomass, in agreement to the results previously reported in the literature (Mendes et al 2006, 2010; Rosa et al 2009; Valladao et al 2011)
The results showed that the hydrolysis and anaerobic biodegradation could be carried out simultaneously, preferentially using low enzyme concentration (0.05 g L-1)

Summary

Introduction

Lipids are important components in wastewater that cause severe environmental pollution (Masse et al 2001, 2003; Mendes et al 2005, 2006, 2010; Demirel et al 2005; Cammarota and Freire 2006; Rosa et al 2009; Valladao et al 2011). Treating wastewater rich in lipids in anaerobic digesters may cause operational problems including the flotation of granular biomass conducting to wash out, and severe toxicity of lipids against both methanogenic and acetogenic microorganisms (Vidal et al 2000; Angelidaki and Sanders 2004) To cope with these negative effects on water stream treatment, several strategies for removing lipid residues at the head of the plant have been proposed such as thermochemical, alkaline and enzymatic pretreatments (Sawayama et al 1995; Masse et al 2001; Mouneimne et al 2003; Mendes et al 2005, 2006, 2010; Cammarota and Freire 2006; Rosa et al 2009; Valladao et al 2011; Alexandre et al 2011). These enzymes showed potential applications in hydrolyzing oil and fats in

Methods

Results

Conclusion