Abstract
A method for the simultaneous determination of yohimbine and boldine in mixtures by first-derivative synchronous spectrofluorimetry has been developed. The method is based on their native fluorescence in 0.1N sulphuric acid medium. The constant wavelength difference chosen to optimize the determination was Δλ=λem -λem=82 nm. Yohimbine was measured at λex//λem= 285/367 nm, and boldine at λex/λem=272/354 nm. The range of application is 10–500 μg/l for yohimbine and 1–50 μg/l for boldine. The method was applied to the determination of yohimbine and boldine in synthetic mixtures and pharmaceuticals, with errors generally ⩽2%. Relative standard deviations were about 2%.
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