Abstract
A simple and sensitive method is proposed for the determination of boldine by first-derivative synchronous spectrofluorimetry based on its native fluorescence in 0.1 N sulphuric acid. The constant wavelength difference ( Δλ = λ cm - λ c chosen to optimize the determination is 90 nm. The linear concentration range of application is between 2.0 and 50.0 wg I- −1 of boldine. the detection limit 0.4 wg l −1 and the relative standard deviation at a concentration of 25 μg l −1 was 2.19%. The method has been satisfactorily applied to the determination of boldine in pharmaceutical formulations.
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