Abstract
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 × 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (μg/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets.
Highlights
Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-high performance liquid chromatographic (HPLC)
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets
Number of high performance liquid chromatographic (HPLC) methods are available for separation and quantiÞcation of valsartan from pharmaceutical dosage forms2
Summary
Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC Et al.: Simultaneous RP-HPLC Determination of Valsartan and Hydrochlorothiazide A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. The retention time (min) and linearity range (μg/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively.
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