Abstract
A simultaneous determination of silicon and phosphorus in biological standard materials with on-line column flow-injection spectrophotometry (FIA) is described. Biological materials are ashed, fused with a lithium carbonate-boric acid mixture, and dissolved in a hydrochloric acid solution. Interfering cations are removed by a simple cation-exchange column filtration. The acid effluent is evaporated to dryness, fused with a small amount of sodium carbonate for depolymerization, taken up in dilute EDTA solution, and analyzed for silica and phosphorus by FIA. For the simultaneous determination of these elements, TSK-gel SAX was used, and the eluent was 0.085 mol/l NaCl/0.01 mol/l NH3/0.001 mol/l EDTA. Several standard reference materials [bovine liver (NBS), chlorella and pepperbush (NIES)] were analyzed for both elements. The results of phosphorus determination for bovine liver are in satisfactory agreement with the NBS certificated value. ICP measurements were applied to analyses of chlorella and pepperbush for silica and phosphorus. The agreement of the analytical results between FIA and ICP is satisfactory. Silica in bovine liver was determined in the present study for the first time.
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