Abstract
The genus Potentilla (Rosaceae) includes about 500 species of perennial, rarely biennial, and annual herbs and small shrubs. Species diversity is highest in northern Eurasia. A few species are also found in high mountain regions of the tropics and South America. In their natural habitat they commonly occur in temperate, arctic and Alpine zones and are also grown for their decorative values [1]. The high content of polyphenolic compounds: tannins, flavonoids, phenolic acids and other active phytochemicals (triterpenes) contributed to their pharmacological effects. Potentilla species are known for their therapeutic properties in the treatment of diarrhoea, dysentery and sore throats and recent investigations have also shown that some extracts of different parts from Potentilla species exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic potential properties [2,3]. The aim of our study was to determinate three polyphenolic compounds: tiliroside (TRS), methyl brevifolincarboxylate (MBR) and ellagic acid (EA) in the diethyl ether extracts obtained from aerial parts of the selected Potentilla species: P. anserina, P. grandiflora, P. nepalensis and P. rupestris by using HPTLC-densitometry method. Chromatography was performed in CAMAG ADC2 (Automatic Development Chamber). HPTLC Silica gel 60 F254 plates were used. As a mobile phase toluene-ethyl formate-formic acid (6:4:1, v/v/v) was used (distance of 7.5cm). Densitometry was carried out by using of Shimadzu CS-9301PC densitometer. The absorption spectra were recorded at 320nm (TRS), 287nm (MBR) and at 280nm (EA). The quantitative amounts of the marker compounds tiliroside, methyl brevifolicarboxylate and ellagic acid ranged from 2.0% to 21.0% in extracts of different Potentilla species. The proposed HPTLC method was found to be simple, precise and accurate for the quantification of these compounds in plant materials.
Published Version
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