Abstract
Simultaneous determination of two antidiabetic drugs, metformin and glyburide, in pharmaceutical tablet formulations were investigated. Normal phase thin layer chromatography plate (silica gel 60 F254) was used as stationary phase and water/methanol/ammonium sulfate (2/1/0.5 w/v) as mobile phase to determine two pharmaceutically active ingredients, in three different formulations of Glucovance®. This system gave a good resolution for metformin (Rf value of 0.43 ± 0.01) and glyburide (Rf value of 0.64 ± 0.02). Determination was by densitometry in the absorbance mode at 237 nm. The linear regression data for the calibration plot showed a good relationship with r = 0.99581 and 0.99982 for metformin and glyburide, respectively. The method was validated for precision and recovery. The limits of detection and quantification were 25.24 and 84.12 ng spot−1 for metformin and 12.26 and 40.86 ng spot−1 for glyburide, respectively. Stability study has been carried out for samples and standard solutions.
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