Simultaneous estimation of amiloride hydrochloride and torsemide in their combined dosage forms by high-performance thin-layer chromatography

Abstract

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of torsemide and amiloride hydrochloride in the pharmaceutical dosage form. Thin-layer chromatographic (TLC) aluminum plates precoated with silica gel 60 F254 were used as the stationary phase, while chloroform-methanol-ammonia (7.5: 3.5: 1, v/v) as mobile phase was used. The RF values observed were 0.46 ± 0.01 and 0.24 ± 0.01 for torsemide and amiloride hydrochloride, respectively. The densitometric analysis was carried out in absorbance mode at 286 nm. The method was linear in the range of 100–600 ng spot−1 for torsemide and 50–300 ng spot−1 for amiloride hydrochloride. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The limit of detection and limit of quantitation were found to be 19.94 ng spot−1 and 100 ng spot−1, respectively, for torsemide. The limit of detection and limit of quantitation were found to be 12.86 ng spot−1 and 50 ng s...