Simultaneous Determination of Lamotrigine and Carbamazepine in Plasma Using Ultrasound-Assisted Emulsification Microextraction-High Performance Liquid Chromatography

Abstract

A simple, fast and efficient method based on ultrasound-assisted emulsification microextraction (USAEME) followed by HPLC has been developed for simultaneous determination of Carbamazepine (CBZ) and Lamotrigine (LTG) in biological samples. The influence of different parameters affecting the USAEME procedure was evaluated to optimize the efficiency of the process. At these conditions, validation of the proposed method was performed based on European medicines agency guidelines. The proposed method showed good linearity in plasma samples ranging from 0.25 to 6 µg/mL and 0.13 to 6 µg/mL (R2 ≥ 0.99) for CBZ and LTG, respectively. The precision of the proposed method was evaluated for repeatability which was ≤12% (n = 5). The detection limits of 0.06 and 0.05 µg/mL were obtained for CBZ and LTG, respectively. The proposed method can be applied as an effective technique for therapeutic drug monitoring of CBZ and LTG in plasma of epileptic patients.