Abstract
Licorice is the most frequently used herb in traditional Chinese medicine (TCM) with versatile functions. It is also a popular natural dietary supplement. While the dosages is very important for there are some side effects caused by licorice. The composition of licorice, its products should be well determined thereof. A simple method for simultaneous determining sixteen compounds in vary high dynamic range of content has been established. This method based on the detection at the characteristic ultraviolet spectra of different types of compounds in licorice. Glycyrrhizin and fifteen flavonoids were well measured. All of these compounds can be precisely quantified at their characteristic wavelengths. This method has been successfully applied to the analyses of different licorices, Sini Tang decoction, and rat plasma after oral administration of Sini Tang decoction. These compounds were found to be over 3000 times in content (from 0.01 μg/g to 34.5 μg/g) in some samples.
Highlights
It is well known that herbal prescription is becoming increasingly popular as supplementary and alternative medicine in recent years
The response range of electrospray ionization ion source (ESI) and some mass analyzers are sometimes not so broadened for quantification of multiple compounds with long dynamic range in contents, whereas in order to understand the mechanism of interactions among the ingredients of licorice during the process of decoction, absorption, metabolism, or at the physiological process, alternations of a series of compounds with similar structure in these processes were needed
A simple HPLC method, based on the different UV characters of triterpenoids, flavones, flavans, and chalcones, for simultaneous determination of glycyrrhizin and 15 flavonoids was developed and validated. Their contents in two wild licorices, prepared licorice, Sini Tang decoction, and the rat plasma after oral administration of Sini Tang decoction were estimated as a proof application
Summary
It is well known that herbal prescription is becoming increasingly popular as supplementary and alternative medicine in recent years. The response range of electrospray ionization ion source (ESI) and some mass analyzers are sometimes not so broadened for quantification of multiple compounds with long dynamic range in contents, whereas in order to understand the mechanism of interactions among the ingredients of licorice during the process of decoction, absorption, metabolism, or at the physiological process, alternations of a series of compounds with similar structure in these processes were needed. A simple HPLC method, based on the different UV characters of triterpenoids, flavones, flavans, and chalcones, for simultaneous determination of glycyrrhizin and 15 flavonoids was developed and validated Their contents in two wild licorices, prepared licorice, Sini Tang decoction, and the rat plasma after oral administration of Sini Tang decoction were estimated as a proof application
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