Simultaneous Determination Of Atenolol And Hydrocholrothiazide In Tablets Formulation By Derivative Spectrometry

Saad Antakli Saad Antakli,Moustafa Alabo Joumaa Moustafa Alabo Joumaa,Leon Nejem Leon Nejem

doi:10.24297/jac.v17i.8808

Saad Antakli Saad Antakli, Moustafa Alabo Joumaa Moustafa Alabo Joumaa + Show 1 more

Open Access

https://doi.org/10.24297/jac.v17i.8808

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Abstract

The derivative spectrophotometric method was developed and applied for the simultaneous determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in Tablets formulations. The first derivative spectrophotometric (1DS) method was applied for the determination of (ATE) and (HCT), respectively. (ATE) was determined at 271.9 nm (1D 271.9) and (HCT) was determined at 279.3 nm (1D 279.3). Linearity showed a good correlation coefficients R2 = 0.9994 and R2 = 0.9989 for (ATE) and (HCT), respectively. Linearity ranges were (10 – 280)

Highlights

Atenolol (ATE) chemically, 4-(2-hydroxy-3-isopropyl aminopropoxy)-phenyl acetamide is a β-adrenoreceptor blocking agent, primarily used in hypertension, angina pectoris and myocardial infraction
Hydrochlorothiazide (HCT), 6-chloro-3, 4-dihydro-2H-1, 2, 4-benzothiadiazine-7-sulfonamide 1, 1-dioxide, which is widely used in antihypertensive pharmaceutical preparations, reduces active sodium reabsorption and peripheral vascular resistance
Chemical regents: Methanol from LOBAL Chemie (INDIA), Hydrochloric acid from SURCHEM PRODUCTS LTD (ENGLAND), Hydrochlorothiazide purity 99.38 % was obtained from China and Atenolol purity 100.42 % was obtained from India, Double distilled water

Summary

Introduction

Atenolol (ATE) chemically, 4-(2-hydroxy-3-isopropyl aminopropoxy)-phenyl acetamide is a β-adrenoreceptor blocking agent, primarily used in hypertension, angina pectoris and myocardial infraction. It mainly acts by inhibition of rennin release and angiotensin-2 & aldosterone production. It is reported to lack intrinsic sympathomimetic activity and membrane-stabilizing properties. Hydrochlorothiazide (HCT), 6-chloro-3, 4-dihydro-2H-1, 2, 4-benzothiadiazine-7-sulfonamide 1, 1-dioxide, which is widely used in antihypertensive pharmaceutical preparations, reduces active sodium reabsorption and peripheral vascular resistance. The review of the literature revealed that no method is yet reported for the simultaneous estimation of both drugs in combined dosage forms. Present work describes two simple, accurate, reproducible, rapid and economical methods for simultaneous estimation of (ATE) and (HCT) in tablets formulation[1]

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